Our team is proud to lead the first randomized ​trial of vaginal microbiome transplant in the ​United States to prevent recurrent BV. Keep ​reading to find out how you can participate in this ​research study as a vaginal fluid donor or as ​someone with recurrent BV!

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Modifying Organisms Transvaginally in Females

BV impacts a third of women ​worldwide, and antibiotic treatment ​fails to provide long term cure in 60% ​of people

Our mi​ssion

This research study will test whether ​transferring vaginal fluid with healthy ​Lactobacillus to someone with recurrent ​bacterial vaginosis will prevent BV from ​coming back.

what is bacterial vaginosis?

Bacterial vaginosis, characterized by a loss of healthy vaginal lactobacillus ​bacteria, impacts close to 30% of US women and is associated with higher risk ​for preterm birth, HIV acquisition, and HPV persistence. In addition, persistent, ​recurrent symptoms can have a significant impact on quality of life. Current ​treatment strategies of vaginal or oral antibiotics fail achieve a durable cure in ​30-60% of women.


  • Antibiotic treatment for BV can provide ​temporary relief, but in up to 60% of ​people, BV comes back within 3-6 ​months.
  • We think this is because the protective ​bacteria, Lactobacillus, do not come ​back after antibiotic therapy.
  • Currently, probiotics available in stores ​have not been widely effective in ​restoring Lactobacillus.

Results of antibiotic treatments

how can you participate?


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trial participants

People 18-55 with > 3 episodes of BV ​in the past year and not in menopause, ​are eligible. Participants:

  • receive oral antibiotics
  • are randomized to 2 doses of ​healthy vaginal fluid or sterile ​placebo.
  • attend a total of 10 visits over 6 ​months.

Participants who complete all study ​visits can receive up to $300.

Healthy people who have never had ​BV, are not in menopause and have ​vaginal Lactobacillus can donate ​vaginal fluid up to 20 times in a 45-​day window. Donors are tested for ​multiple kinds of infections 3 times ​during that window. Participants who ​complete all study visits can receive ​up to $800.

Frequently Asked Questions about ​Vaginal Microbiome Transplant

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How do you know ​vaginal fluid donations ​are safe?

Donors are tested for HIV, Hepatitis (A, B, ​C), Herpes, Syphilis, HPV, CMV ​(cytomegalovirus), HTLV (human T ​lymphotrophic virus), Mono (Epstein Barr ​virus), gonorrhea, chlamydia, trichomonas, ​yeast, mycoplasma before and after ​donating, and for the viral infections a month ​after finishing donation.

We also test each donation for semen, HPV ​and Lactobacillus crispatus .

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How do people ​donate vaginal ​fluid?

Donors place a disposable ​menstrual cup in the vagina the ​night before coming to clinic. ​When they arrive, they remove ​the menstrual cup, and we collect ​the vaginal fluid from that. Donors ​do not donate when menstruating.

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How do you do the ​vaginal fluid ​transplant?

The clinical provider does a ​speculum exam and uses a sterile ​eyedropper to place the vaginal ​fluid (or placebo saline) in the ​participant’s vagina. The speculum ​is then removed and the participant ​lies flat for about 15 minutes. Each ​participant will get 2 doses, 48-72 ​hours apart.

Dr. Caroline Mitchell, MD, MPH ​Principal Investigator

our team

Dr. Douglas Kwon, MD, PhD

Principal Investigator

Daniel Worrall, NP

Clinical Operations Manager

Meena Murthy, CNP

Co-investigator, Clinician

Fatima Hussain

Postdoctoral Fellow

Ankita Katukota

Clinical Research Coordinator

Judith Brister

Clinical Research Coordinator

Agnès Bergerat-Thompson, PhD ​Senior Laboratory Technologist

Briah Cooley Demidkina

Senior Clinical Research Coordinator

Wafae El-arar

Laboratory Technician

Miles Goldenberg

Laboratory Technician

Aditi Kannan

Research Technician

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Natalie Culler

Research Technician

Jiawu Xu

Se​nior Research Scientist

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Get in touch

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Potential participants contact:

Ankita Katukota

Clinical Research Coordinator

(857) 268-7068

Judith Brister

Clinical Research Coordinator

(857) 268-7065

For clinical questions contact:

Dr. Caroline Mitchell

Principal Investigator

(617) 724-3326

Dr. Douglas Kwon

Principal Investigator

(857) 268-7160

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